Efficient extraction of genomic DNA (gDNA) from biological materials found in harsh environments is the first step for successful forensic DNA profiling. This study aimed to evaluate two methods for DNA recovery from animal tissues (livers, muscles), focusing on the best storage temperature for DNA yield in term of quality, quantity, and integrity for use in several downstream molecular techniques. Six male Swiss albino mice were sacrificed, liver and muscle tissues (n=32) were then harvested and stored for one week in different temperatures, -20C, 4C, 25C and 40C. The conditioned animal tissues were used for DNA extraction by Chelex-100 method or NucleoSpin Blood and Tissue kit. The extracted gDNA was visualized on 1.5% agarose gel electrophoresis to determine the quality of gDNA and analysed spectrophotometrically to determine the DNA concentration and the purity. Both methods, Chelex-100 and NucleoSpin Blood and Tissue kit found to be appropriate for yielding high quantity of gDNA, with the Chelex100 method yielding a greater quantity (P < 0.045) than the kit. At -20C, 4C, and 25C temperatures, the concentration of DNA yield was numerically lower than at 40C. The NucleoSpin Blood and Tissue kit produced a higher (P=0.031) purity product than the Chelex-100 method, particularly for muscle tissues. The Chelex-100 method is cheap, fast, effective, and is a crucial tool for yielding DNA from animal tissues (livers, muscles) exposed to harsh environment with little limitations.
Huda H. Al-Griw, Zena A. Zraba, Salsabiel K. Al-Muntaser, Marwan M. Draid, Aisha M. Zaidi, Refaat M. Tabagh , Mohamed A. Al-Griw(8-2017)

Abstract Biological studies of Nezara viridula (L.) (Heteroptera:Pentatomidae) and its parasitoid Trissolcus basalis (Wollaston)(Scelionidae: Hymenoptera) collected from Libya were conducted in a rearing room at 25 ± 5°C, 65 ± 10 % R.H. and 16:8 LD photoperiod.During the course of rearing of N. viridula the eggs incubation periodvaried between 4-7 days. The newly emerged nymphs and the 5th stage had the highest mortality in comparison with 1st, 2nd, 3rd and 4thstages .studies on T. basalis included: SEM, development time, parasitoid mating behavior and oviposition behavior, functionalresponse and fecundity. The development time was 4 to 10 days underthe laboratory condition. Adult males lived longer than females.Mating time took from 12.2 to 19.5 seconds. In case of ovipositionbehavior serial of behaviors were observed and recorded. The egg selection period was 0: 00: 08 - 0: 00: 25 min, oviposition period was 0: 01: 04 - 0: 01: 41 min. Egg marking period was 0: 00: 11 - 0: 00: 25 min. The longest period was the oviposition period followed by egg marking period followed by egg selection period.In functional response study was found a highly positive correlation ( r = 0.974 ) number of egg masses exposed and the number of parasitoid emerged. Fecundity experiments showed that the daily ii production of progeny by female of T. basalis during 6-10 dayslifetime showed that female progeny production was higher than male progeny production. The maximum period offspring production was 6-10 days. There was a highly negative correlation ( r = - 0.900 ) between the number of eggs parasitized and parasitoid age . Also there is a highly signifcant difference between the fecundity of T. basalis newly emerged of 24 h and after 7 days after emergence from N. viridula eggs. The number of mature eggs in the ovary were less in 24h aged female and the fecundity increased over the next 7 days.
عايدة عادل عبد الرحيم بادي (2008)

الملخص أظهرت النتائج الشهرية باستخدام أقماع بيرليزي وجود اختلافات في الكثافة العددية للحيوانات الدقيقة المرتبطة بأشجار الأيوكالبتوس أو الكافور Eucalyptus camaldulensis والصنوبر Pinus halepensis والعوامل المناخية في غابة النصر بطرابلس لإجمالي 021 عينة من نثار الأوراق لسنة 2112 . بلغ إجمالي الكثافة العددية للحيوانات الدقيقة السنوية 002.111 فردًا / 01 cm / m من نثار الأوراق. أوضحت النتائج أن الكثافة العددية للكوليمبولا كان فعالاً في تفكك ³ وتكسير نثار الأوراق، والحلم كمفترسات، والفطريات والبكتيريا لتحلل نثار الأوراق. تم تقسيم الدراسة إلى فترتين. ) 0( مايو - 2، والكوليمبولا 2.، والحيوانات الدقيقة الأخرى 011 فرد/ .. أكتوبر، أظهرت أن متوسط وفرة الحلم كان 2 01 cm / m ³ من نثار الأوراق على التوالي. ) 2( نوفمبر- أبريل، أظهرت أن متوسط وفرة الحلم كان 21 ، والكوليمبولا 135 ، ومجموعة الحيوانات الدقيقة الأخرى 011 فرداً / 15 cm / m نثار الأوراق على التوالي. أوضحت الدراسة وجود فروق ذات دلالة ³ معنوية عند المستوى 1.110 وفي اختبار Duncan عند المستوى 1.110 بين شهر يناير والأشهر الأخرى. تم عرض جميع البيانات البيولوجية والكيميائية والفيزيائية المؤثرة على التنوع البيولوجي لمفصليات الأرجل ونثار أوراق الكافور والصنوبر في غابة النصر بطرابلس. توصي الدراسة بالنظر في أهمية تعريف وتصنيف والتنوع البيولوجي للحيوانات الدقيقة والعوامل المختلفة المرتبطة بنثار الأوراق كمؤشرات بيئية مهمة واستدامة النظام البيئي للغابات في ليبيا.
هدي مصطفي عمر الشبة , نوال عبد السلام محفوظ(12-2021)