virus infection continues to be an important public health concern with regard to blood transfusion in Libya and in Africa. This concern is related to the screening test. Objectives: The main aim of this study to investigate the blood donors samples for HIV, HBV and HCV infections in Tripoli-Libya, North Africa during the first five months of 2015. Methods: The total of 686 blood samples obtained from healthy blood donors who attended Tripoli’s central blood bank, were tested for HBsAg, HCV and HIV using the VITROS® 3600 Immunodiagnostic System. Results: From the 686 samples examined, the frequency of HBsAg positive cases was 0.8%, the number of anti-HBc positive samples was found to be particularly high in the age group 29 and 36 years (p= 0.0001). The number of anti-HBc positive samples was found to be particularly high in the age group 30-39 years (p= 0.01). Most occupation that had positivity with anti-HBc and HBV-DNA were free workers and was less in students. Most positive cases were from east of Tripoli the capital (Tagora, Soq-Aljomaha). Conclusion: The frequency of HBsAg positive blood donors and anti-HBc among this sample was 0.8% and 0.7% respectively, which is low compared with the international findings. The current study estimated the expected exclusion rate of anti-HBc and HBsAg positive donated blood, as this would be an important factor to consider before donation. arabic 16 English 63
Basma Mohamed K Doro(9-2015)

Leishmaniasis is a major health problem and itis estimated that 12 million people are currently infected.A vaccine which could cross-protect people against different Leishmania spp. would facilitate control ofthis disease as more than one species of Leishmania may be present. In this study the ability of a DNA vaccine, using the full gene sequence for L. donovani gamma glutamyl cysteine synthetase (GCS) incorporated in the pVAX vector (pVAXGCS), and a protein vaccine, using the corresponding recombinant L. donovani GCS protein (LdGCS),to protect against L. major or L. mexicana infection was evaluated. DNAvaccination gave transient protection against L. major and no protection against L. mexicana despite significantly enhancing specific antibody titres in vaccinated infected mice compared to infected controls. Vaccination with the LdGCS protected against both species but only if the protein was incorporated into non-ionic surfactant vesicles for L. mexicana. The results of this study indicate that a L. donovani GCS vaccine could be used to vaccinate against more than one Leishmania species but only if the recombinant protein is used. arabic 30 English 176
Basma Doro, (12-2012)

Aims: The aim of the study was to assess the antibacterial effect of Ficus carica extracts against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, & Staphylococcus aureus. Study Design: Evaluation of antimicrobial activity using Cup-cut agar method. Place and Duration of Study: Microbiology Research Laboratory, Department of Microbiology and Immunology, Faculty of Pharmacy, University of Tripoli, from October 2015 to March 2016. Methodology: The leaves and stem part extracts of Ficus carica plant were prepared using maceration method. The antibacterial activities of the extracts were evaluated using Cup-cut agar method to determinate inhibitory zone diameters in millimeters of the plant extracts against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. The measurement of exponential bacterial growth curves was used to determine the type of growth pattern spectrophotometrically at 600 nm. Furthermore, plate count methods were also used to enumerate the bacterial count and to determine the percentage of inhibition as well as IC50. Results: The results of this study showed that Ficus carica extracts used, exhibited antimicrobial activity against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. The maximum zone of inhibition against S. aureus (27± 0.04mm, p
Basma Mohamed K Doro(1-2018)