Vibrio vulnificus, a zoonotic bacterial pathogen was isolated for the first time from kidneys of black scorpionfish Scorpaena porcus collected from the south Mediterranean basin (western coast of Tripoli, Libya) during the early summer of 2013. Fish exhibited typical signs of Vibriosis with furuncle like lesions, skin hemorrhages, fin rot and congested spleen. The abdominal cavity, liver, intestinal wall, and gonads were suffering from mild larval nematode infection. Interestingly, a huge portion of the kidney tissue was replaced with the anisakids nematode Anisakis pegreffi. This is considered the first report ofV. vulnificus / A. pegreffi in the Mediterranean black scorpionfish. The identities of the retrieved V. vulnificus isolates were confirmed using morpho-chemical and molecular tools. The research lead us to conclude that the deteriorated nature of the sewage polluted seawater at the western coast of Tripoli could have played a detrimental role in facilitating the invasion with the two diverse etiological agents (V. vulnificus and A.pegreffi) . We also hypothesize that V. vulnificus was lodged within nematode worms or at one of its life stages before invading fish intestine with consequent spread into other internal organs during its visceral larval migration stage.
Abdulwahab Kammon(5-2015)

Infectious Laryngotracheitis (ILT) is a viral respiratory disease of poultry caused by Gallid herpesvirus 1 leading to severe economic losses. The aim of the current study was to isolate and identify ILT virus in suspected outbreaks of one broiler flock (farm 1) and three layer flocks (farms 2,3 and 4) located in Tripoli. Samples of trachea were collected and preserved in transport media for viral isolation and some were preserved in 10% neutral formalin for histopathology. Broiler and Layer flocks were serologically monitored and tested for the presence of antibodies by ELISA against ILTV at first days old and later at slaughter age for broilers and before the onset of production for layers in which total of 290 blood samples were collected from six poultry farms (3 broiler flocks and 3 layer flocks). Following isolation of the virus in fertile eggs, chorio-allantoic membrane (CAM) samples were confirmed by PCR and histopathology. The lesions seen on the CAMs were congestion, hemorrhages and pocks after 5 days post inoculation. Samples from farm 1 and 3 were positive whereas other farms were negative. Histopathology of CAMs revealed edema, congestion, haemorrhages, infiltration of heterophils and mononuclear cells, and presence of syncytial cells containing intranuclear inclusion bodies. The trachea revealed severe destruction and sloughing of the mucosa due to necrosis. The results of this study confirmed the circulation of ILTV in commercial poultry flocks and highlighted the need for more epidemiologic investigation of the disease arabic 16 English 96
Abdulwahab Kammon, Jamaal Shabba, Yousef Abouzeed, Abdulatif Asheg (2-2020)

Abstract The use of antibiotics as growth promotors to enhance animal production is banned in many countries around the world due to antimicrobial resistance. There is a need therefore, for new alternatives to antibiotics in medicine and veterinary medicine practices. The antibacterial activity of clove oil and some antibiotics were tested in vitro against three isolates of E. coli {1 avian pathogenic (E. coli 6.2) and 2 non-pathogenic (E. coli 6.1 and E. coli X)}, Salmonella enteritidis and Salmonella spp., by disk diffusion and minimum inhibitory concentration (MIC) methods. All bacteria tested showed intermediate susceptibility to clove oil using disk diffusion method except the non-pathogenic E.coli 6.1. The inhibition zones measured were 0mm, 12mm, 13mm, 15mm and 15mm for E. coli 6.1, E. coli 6.2, E. coli X, Salmonella enteritidis and Salmonella spp., respectively. E. coli 6.1 was resistant to Ampicillin, and Lincomycin with multi antibiotic resistance (MAR) index of 0.2. E. coli 6.2 was resistant to Ampicillin, Doxycyclin and Lincomycin with MAR index of 0.3. E. coli X was resistant to Ampicillin and Colistin with MAR index of 0.2. Salmonella enteritidis was resistant to Amoxycillin/Clavulinic acid, Ampicillin and Lincomycin with MAR index of 0.3. Salmonella spp was susceptible only to Neomycin but was resistant to 9 out of 10 antibiotics with very high MAR index of 0.9. The MICs of clove oil were 6.25mg/ml for the avian pathogenic E. coli and 3.12mg/ml for non-pathogenic E. coli and Salmonella spp. and 12.5mg/ml for Salmonella enteritidis. It is concluded that clove oil has promising antibacterial activity and more studies are needed to investigate its in vivo activity as alternative to antibiotics in poultry arabic 13 English 85
Abdulwahab Kammon, Ali Almaeyoufi , Abdulatif Asheg(7-2019)